|
KMID : 0903520040470040430
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 4 p.430 ~ p.433
|
|
|
Utilization of Pollen Grain from Liluim cv. Casablanca as a Transient Gene Expression Host
|
|
Park Hee-Sung
|
|
|
Abstract
|
|
|
|
Lilium cv. Casablanca pollen grains stored at ?70oC were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at 27oC. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of 200 μM acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.
|
|
|
KEYWORD
|
|
|
Lilium cv, Casablanca, pollen, Agrobacterium, transformation, GUS histochemistry
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|